Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Humoral immune responses following vaccination Antibody responses at weeks 0 (baseline), 8 (post-prime), 14 (pre-boost), and 18 (post-boost) following vaccination with BNTx3, BNTx2/Ad26, Ad26/BNT, Ad26x2, or sham (N = 30; N = 6/group). (A) Neutralizing antibody (NAb) titers by a luciferase-based pseudovirus neutralization assay. (B) Receptor-binding domain (RBD)-specific binding antibody titers by ELISA. Responses were measured against the SARS-CoV-2 WA1/2020 (black), B.1.617.2 (Delta; blue), B.1.351 (Beta; red), and B.1.1.529 (Omicron; green) variants. Dotted lines represent limits of quantitation. Medians (red bars) are shown. Omicron-specific NAbs in the vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Luciferase, Neutralization, Binding Assay, Enzyme-linked Immunosorbent Assay, Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Cellular immune responses following vaccination T cell responses at weeks 14 (pre-boost) and 18 (post-boost) following vaccination with BNTx3, BNTx2/Ad26, Ad26/BNT, Ad26x2, or sham (N = 30; N = 6/group). (A and B) Pooled peptide spike-specific IFN-γ (A) CD8+ T cell responses and (B) CD4+ T cell responses by intracellular cytokine staining assays. Responses were measured against the SARS-CoV-2 WA1/2020 (black), B.1.617.2 (Delta; blue), and B.1.1.529 (Omicron; green) variants. Dotted lines represent limits of quantitation. Medians (red bars) are shown. Omicron-specific CD8+ and CD4+ T cell responses in the vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Staining, Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Viral loads following SARS-CoV-2 Omicron challenge (A) Log subgenomic RNA (sgRNA) copies/mL in bronchoalveolar lavage (BAL) following SARS-CoV-2 Omicron challenge. (B) Log subgenomic RNA (sgRNA) copies/swab in nasal swabs (NS) following SARS-CoV-2 Omicron challenge. Medians (red lines) are shown.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques:

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Comparison of peak and day 4 viral loads (A) Log subgenomic RNA (sgRNA) copies/mL in bronchoalveolar lavage (BAL) at peak and on day 4 following SARS-CoV-2 Omicron challenge. (B) Log subgenomic RNA (sgRNA) copies/swab in nasal swabs (NS) at peak and on day 4 following SARS-CoV-2 Omicron challenge. Dotted lines represent limits of quantitation. Medians (red bars) are shown. Vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: TCID50 titers, related to Figure 3 Log TCID50/mL in bronchoalveolar lavage (BAL) and nasal swabs on day 2 following SARS-CoV-2 Omicron challenge (top). Log TCID50/mL is also shown in nasal swabs on day 7 following SARS-CoV-2 Omicron challenge in the 4 vaccinated animals in the BNTx3 and BNTx2/Ad26 groups and in the 6 sham controls with persistently positive sgRNA levels on day 7 (bottom). Dotted lines represent limits of quantitation. Medians (red bars) are shown. Vaccinated groups were compared with the sham controls by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Quantitation Assay, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet: Histopathology and immunohistochemistry of Omicron infection, related to Figure 1 (A–K) (A–C) Pharynx and (D–K) lungs from macaques on day 2 following Omicron infection demonstrated lymphoid hyperplasia of the pharynx (A and B), SARS-N positive ciliated epithelial cells in the pharynx (C), foamy macrophages and degenerating neutrophils in bronchiole lumen (D), cellular necrotic debris adhering to bronchiolar ciliated epithelium (E), alveolar syncytia (F), SARS-N-positive ciliated epithelial cells in the pulmonary interstitium (G), neutrophilic bronchitis (H), hyaline membranes (I), endothelialitis (J), and type II pneumocyte hyperplasia (K). Scoring involved assessment of the following lesions: interstitial inflammation and septal thickening, interstitial infiltrate (eosinophils), interstitial infiltrate (neutrophils), hyaline membranes, interstitial fibrosis, alveolar infiltrate (macrophages), bronchoalveolar infiltrate (neutrophils), epithelial syncytia, type II pneumocyte hyperplasia, bronchi infiltrate (macrophages), bronchi infiltrate (neutrophils), bronchi (hyperplasia of bronchus-associated lymphoid tissue), bronchiolar or peribronchiolar infiltrate (mononuclear cells), perivascular infiltrate (mononuclear cells), and endothelialitis. Each feature assessed was assigned a score of: 0, no substantial findings; 1, minimal; 2, mild; 3, moderate; 4, moderate to severe; 5, marked or severe. Scores were added for all lesions across all lung lobes for each macaque, for a maximum possible score of 600 for each macaque. (L) Summary of lung pathology scores from SARS-CoV-2 WA1/2020- and Omicron-infected macaques. Medians (red bars) are shown. Dotted line represents no pathology. Lung pathology scores were compared in macaques infected with Omicron versus WA1/2020 by two-sided Mann-Whitney tests. ∗ p < 0.05.

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Histopathology, Immunohistochemistry, Infection, MANN-WHITNEY

Journal: Cell

Article Title: Vaccine protection against the SARS-CoV-2 Omicron variant in macaques

doi: 10.1016/j.cell.2022.03.024

Figure Lengend Snippet:

Article Snippet: SARS-CoV-2 (Omicron) RBD , Sino Biological , Cat # 40592-V08H121.

Techniques: Infection, Recombinant, Binding Assay, Blocking Assay, Software